Cytometry is translated as the measurement of cells, where cells are the basic biological unit of all organisms.
Flow cytometry is a specialised laser-based single cell technology used to measure different parameters of a particle (cell) flowing in suspension. The scattered and fluorescent light is collected by detectors and converted to electronic signals for real time analysis within dedicated software.
Fluorescence activated cell sorting (FACS) instruments utilise this technology to yield separated and purified populations of interest or single cells suitable for downstream applications, such as functional assays or genomic and transcriptomic analyses.
IRR Cell Sorting and Flow Cytometry Facility
Based in: Institute for Regeneration and Repair (IRR)
Facility manager/ lead contact: Ms Fiona Rossi, Dr Claire Cryer and Ailsa Laird
This facility provides comprehensive services and assistance in multi-parameter flow cytometry analysis, fluorescence activated cell sorting (FACS) for internal and external users.
Facility users are trained to independently use the facilities: three high-end flow cytometry analysers. The cell sorting service is operated by experienced cytometrists on three available cell sorters. The team can sort up to four populations simultaneously and has extensive experience with 384- and 96-well plate and index sorting.
IRR Cell Sorting and Flow Cytometry Facility website
IGC Flow cytometry facility
Based in: Institute of Genetics and Cancer (IGC)
Facility manager/ lead contact: Ms Elisabeth Freyer
The facility is managed and operated by an experienced Flow Cytometry Manager with more than 10 years of experience and can be accessed by all Institute of Genetics and Cancer researchers and their collaborators.
The facility operates 2 flow cytometry analysers and 2 flow cytometry sorters. The cost of accessing the facility is maintained in line with the average cost of other University of Edinburgh flow cytometry facilities.
IGC Flow Cytometry Facility website