Host and tumour profiling unit (HTPU): microarray services
Based in: Institute of Genetics & Cancer (IGC)
Facility manager/ lead contact: Alison Munro and Kenneth Macleod
This service provides protein, antibody and transcriptomic microarray facilities for the analysis of complex signalling pathways and secreted factors across multiple pre-clinical and clinical sample types:
- NanoString nCounter - highly sensitive profiling of hundreds of mRNAs, microRNAs, SNVs, CNVs or protein using colour-coded molecular barcodes to provide a digital count of target molecules. Optimised for difficult sample types including FFPE, tissue and lysate samples.
- Reverse phase protein arrays (RPPA) - a sensitive antibody based proteomic approach enabling simultaneous quantification of multiple proteins and post-translational modifications. Our portfolio of antibodies covers key cancer survival, cell cycle and canonical signalling pathways that can be used to profile multiple pathway responses.
- Forward phase antibody microarrays (cytokine arrays) - utilises well-validated capture/detection antibody pairs for the relative quantification of secreted factors associated with inflammation and angiogenesis i.e chemokines/cytokine/adipokines.
These facilities are available to all University staff, and to external groups through funded collaboration or fee-for-service contract. The NanoString service obtained ACCORD Accreditation in 2021 and can analyse locally sponsored clinical trial samples.
HTPU Microarray Services website
Single-cell multi-omics
Based in: Centre for Regenerative Medicine (CRM)
Facility manager/ lead contact: Dr Andrea Corsinotti
The facility is equipped with a 10x genomics chromium controller that allows us to generate single cell genomic and transcriptomic data (RNA-seq, ATAC-seq, etc.) from up to 10,000 cells and from multiple samples in parallel, and can offer a fully supported sample preparation service: from cells to libraries that includes QC, quantification and lab consumables.
Single-cell multi-omics facility website
Mass spectrometry for metabolomics, lipidomics, proteomics and steroidomics
Untargeted analysis using dedicated pipelines, high resolution mass spectrometry and data interpretation enable metabolomic, lipidomic and proteomic discoveries.
- Metabolomics, proteomics and lipidomics are carried out at the IGC facility, led by Dr Alex von Kriegsheim.
Institute for Genetics and Cancer Mass Spectrometry Facility
- Steroid profiling using targeted or untargeted analysis in clinical and preclinical studies for Steroidomics: Queen's Medical Research Institute (QMRI) Mass Spectrometry Facility in the Centre for Cardiovascular Science (CVS), led by Dr Natalie Homer.
QMRI Mass Spectrometry Facility in CVS
- Proteomics and metabolomics: Roslin Mass Spectrometry Facility with Dr Dominic Kurian.
Roslin Mass Spectrometry Facility
Genomics at Edinburgh
Genomics at Edinburgh is a network and hub for all genomics research at the University, with a comprehensive website showcasing all genomics research and related facilities across campuses.